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Conversion of calcidiol to its biologically active form, calcitriol, occurs through the activity of a specific 25-hydroxyvitamin D 3 1-α-hydroxylase (commonly just called 1-α-hydroxylase) present in the inner mitochondrial membrane of cells of the proximal convoluted tubules of the kidneys, and in several extrarenal sites such as in keratinocytes, macrophages, and placenta