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After cells were cultured for one week, DMEM with 30 μg/mL of 13 C 5 methionine (Cambridge Isotope Laboratories, CLM-893-H) was added to the cells for six hours before rinsing three times with sterile PBS, and lysis was performed using the previously mentioned protocol
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In addition, the sedative effect of the compound preparation of Qishiwei Zhenzhu pills may be related to the efficacy of pearls, but due to the large number of medicinal materials contained in the preparation, the role of pearls in this preparation still needs to be explored
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